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Reciprocal Alterations in Osteoprogenitor and Immune Cell Populations in Rheumatoid Synovia (CROSBI ID 322259)

Prilog u časopisu | pregledni rad (stručni) | međunarodna recenzija

Barbarić Starčević, Katarina ; Lukač, Nina ; Jelić, Mislav ; Šućur, Alan ; Grčević, Danka ; Kovačić, Nataša Reciprocal Alterations in Osteoprogenitor and Immune Cell Populations in Rheumatoid Synovia // International journal of molecular sciences, 23 (2022), 20; 12379, 10. doi: 10.3390/ijms232012379

Podaci o odgovornosti

Barbarić Starčević, Katarina ; Lukač, Nina ; Jelić, Mislav ; Šućur, Alan ; Grčević, Danka ; Kovačić, Nataša

engleski

Reciprocal Alterations in Osteoprogenitor and Immune Cell Populations in Rheumatoid Synovia

: Rheumatoid arthritis (RA) is chronic, autoimmune joint inflammation characterized by irreversible joint destruction. Besides increased resorption, destruction is a result of decreased bone formation, due to suppressed differentiation and function of the mesenchymal lineage-derived osteoblasts in inflammatory milieu. In this study, we analyzed the cellular composition of synovial tissue from 11 RA and 10 control patients harvested during planned surgeries in order to characterize resident synovial progenitor populations. Synovial cells were released by collagenase, and labeled for flow cytometry by two antibody panels: 1. CD3- FITC, CD14-PE, 7-AAD, CD11b- PECy7, CD235aAPC, CD19-APCeF780 ; and 2. 7-AAD, CD105-PECy7, CD45/CD31/CD235a-APC, and CD200- APCeF780. The proportions of lymphocytes (CD3+ , CD19+ ) and myeloid (CD11b+ , CD14+ ) cells were higher in synovial tissue from the patients with RA than in the controls. Among non-hematopoietic (CD45−CD31−CD235a−) cells, there was a decrease in the proportion of CD200+CD105− and increase in the proportion of CD200−CD105+ cells in synovial tissue from the patients with RA in comparison to the control patients. The proportions of both populations were associated with inflammatory activity and could discriminate between the RA and the controls.

rheumatoid arthritis ; osteoprogenitors ; flow cytometry

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Podaci o izdanju

23 (20)

2022.

12379

10

objavljeno

1422-0067

10.3390/ijms232012379

Trošak objave rada u otvorenom pristupu

Povezanost rada

Temeljne medicinske znanosti

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