engleski

Effect of quinazolinones derivates on greater wax moth (Galleria mellonella L.)

of biological activities. Ten quinazolinones based Schiff bases, newly synthesized were tested for their insecticidal activities against greater wax moth (Galleria mellonella L.). Oral bioassay was conducted on last stage insect larvae, which were fed with the control diet or the diet containing 100 mg of tested compound per kg of diet. Mortality, change in behavior (orientation test), symptoms on cuticle (change in color, lesions and spots), and effects on molting were monitored after 24, 48 and 72 h. The compounds did not cause mortality in insects, or change in insect behavior. Larvae after turning on its back were able to return to upright position till pupation. After 24 h, eight compounds induced changes on insect cuticle. The greatest number of larvae exhibited change in cuticular color after 48 h. Compound SB17 caused brown and black spots on the cuticle in highest number of insects in each monitoring period. After 72 h, all compounds induced molting to pupa (min 3, 33% to max 60% larvae pupated), compared to the control where no pupae were observed. Compounds SB3, SB8, SB14, SB21, SB17, SB19 and SB25 induced earlier pupation in min 40% larvae. The highest and statistically significant effect on earlier pupation was observed for compound SB3, which induced molting to pupa in min 60% of larvae after 72 h. Molting starts after juvenile hormone secretion drops in the last larval instar. Compounds SB3, SB8, SB14, SB21, SB17, SB19 and SB25 should be further tested for their activity as insect growth regulators.

Schiff bases, insect mortality, insect behavior, cuticular changes, molting

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