Impact of Deoxynivalenol and Zearalenone as Single and Combined Treatment on DNA, Cell Cycle and Cell Proliferation in HepG2 Cells (CROSBI ID 321987)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Domijan, Ana-Marija ; Hercog, Klara ; Štampar, Martina ; Gajski, Goran ; Gerić, Marko ; Sokolović, Marijana ; Žegura, Bojana
bilateralni projekt, BI-HR/20-21-019
engleski
Impact of Deoxynivalenol and Zearalenone as Single and Combined Treatment on DNA, Cell Cycle and Cell Proliferation in HepG2 Cells
The study aimed to investigate toxicity and the mechanism of toxicity of two Fusarium mycotox-ins, deoxynivalenol (DON) and zearalenone (ZEA). DON and ZEA were applied to HepG2 cells as single compounds and in combination at low environmentally relevant concentrations. HepG2 cells were exposed to DON (0.5, 1, and 2 µM), ZEA (5, 10, and 20 µM) or their combinations (1 µM DON + 5 µM ZEA, 1 µM DON + 10 µM ZEA and 1 µM DON + 20 µM ZEA) for 24 h and cell viability, DNA damage, cell cycle and proliferation were assessed. Both mycotoxins reduced cell viability, however, combined treatment with DON and ZEA resulted in higher reduction of cell viability. DON (1 µM) induced primary DNA damage, while DON (1 µM) in combination with higher ZEA concentrations showed antagonistic effects compared to DON alone at 1 µM. DON arrested HepG2 cells in G2 phase and significantly inhibited cell proliferation, while ZEA had no significant effect on cell cycle. The combined treatment with DON and ZEA arrested cells in G2 phase to a higher extend compared to treatment with single mycotoxins. Potentiating effect observed after DON and ZEA co-exposure at environmentally relevant concentrations indicates that in risk assessment and setting governments’ regulations, mixtures of mycotoxins should be considered.
mycotoxins ; comet assay ; flow cytometry ; co-exposure ; food monitoring
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Podaci o izdanju
24
2023.
4082
13
objavljeno
1422-0067
10.3390/ijms24044082
Trošak objave rada u otvorenom pristupu
Povezanost rada
Biologija, Farmacija, Javno zdravstvo i zdravstvena zaštita