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Optimizing the method omitting cell lysis and protein extraction for identification of bacteria from positive blood cultures by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

In comparison to similar protocols omitting cell lysis and protein extraction, we demonstrated a similar performance for the identification of gram-negative and superior performance for the identification of Gram-positive bacteria from positive blood cultures. This could be explained by including both the additional spin step and wash step in the procedure. Although in-house protocols aare fast, easy and inexpensive, extraction with methods such as Sepsityper is the solution if we want to provide a greater rate of precision for the identification of gram-poitive bacteria.

MALDI-TOF mass spectrometry ; blood culture ; sepsis

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