engleski

Supercritical CO2 extraction of phenolic compounds from medicinal plants

The usage of environmentally friendly extraction techniques for the discovery of novel synthetic compounds available in medicinal plants has a high priority with everyday advances in the field of pharmaceuticals. In this research, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The obtained extraction yield was the highest for the chamomile (5%) sample. The chemical composition analyzed by gas chromatography-high-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1, 1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2=0.818) and ferric reducing antioxidant power (FRAP) assay (R2=0.640), but not to the total phenolic content (R2=0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest.

plants ; square-wave voltammetry ; liquid chromatography ; gas chromatography ; mass spectrometry

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