engleski

Croatian traditional apple varieties as a source of bioactive compounds

Features that make traditional apple varieties interesting for researchers are their polyphenol content, especially phenolic acids, anthocyanins, dihydrochalcones, flavonols and flavan-3-ols [1]. Twenty-three Croatian traditional apple varieties were extracted from dried fruit using 80% aqueous methanol and analysed by HPLC-DAD and LC-MS/MS. Total polyphenol content (TPC) was measured by an optimized Folin-Ciocalteu assay and antioxidant activity was determined by the DPPH method. The TPC content varied from 326.49 mg/100 g to 378.15 mg/100 g dw depending on the varieties. The sum of polyphenols in apple samples ranged from 9.6 mg/kg to 1243.8 mg/kg dw. LC-MS/MS allowed the identification and quantification of eight compounds, namely procyanidin B1, procyanidin B2, catechin, epicatechin, quercetin-3-ß-glucoside, quercetin-3-rutinoside, astragalin and chlorogenic acid. The main polyphenol in all apple samples was chlorogenic acid with content ranging from 1.3 mg/kg dw to 455.9 mg/kg dw, the highest content of chlorogenic acid had ˝Princeza˝.This work was supported by the project UIP-2020-02-8461 (The Croatian Science Foundation).

traditional apple varities ; bioactive compounds ; polyphenols ; chlorogenic acid

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