engleski

Genetic stability of recombinant mumps viruses generated by reverse genetics technology

Recombinant mumps viruses (MuVs) based on established vaccine strains represent attractive vector candidates as they have track records for high efficacy. We developed a rescue system based on the consensus sequence of L-Zagreb vaccine and generated seven different recombinant MuVs. In primary rescued stocks, low percentages of heterogeneous positions were found (maximum 0.12%) and substitutions were predominantly obtained in minor variants, with maximally four substitutions seen in consensus (relative to input plasmids). Still, during the virus stock generation, sub- consensus viral variability should be closely monitored. As we show for Pro408Leu mutation in the L gene, strongly positively selected variants can rise to frequencies over 85% in only few passages (1-4 in our experiments). Six substitutions were characteristic for recombinant viruses generated in our system ; they repetitively occurred during the rescue processes, but their significance remains unknown.

recombinant mumps viruses, rescue, genetic variability

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