engleski

Antibacterial properties of chitosan/poly(acrylic acid) polyelectrolyte multilayer with adsorbed lysozyme layer

Surface properties are crucial in controlling the adhesion of bacteria to materials. The most important properties for adhesion are roughness, charge and hydrophilicity. [1, 2] In this study, we characterized the surface of Si/SiO 2 substrate covered by biocompatible polyelectrolyte multilayer (PEM) made of chitosan (CS) and poly(acrylic acid) (PAA). Furthermore, we investigated the antibacterial properties of (CS/PAA) multilayer film with an added terminating lysozyme layer. The aforementioned properties were examined and characterized with a wide array of methods. The surface charge properties were obtained by zeta potential measurements, while the surface roughness was characterized by atomic force microscopy (AFM). The thickness of the multilayers was determined by AFM and ellipsometry. The hydrophilicity of the surface was investigated using the tensiometer. After the adhesion of bacteria Pseudomonas aeruginosa unmodified and lysozyme-modified PEMs, surface was studied using scanning electron microscope (SEM). Many of the aforementioned properties directly influence the extent of the adhesion of bacteria. These properties vary with different terminating polyelectrolyte layers and with the presence of the adsorbed lysozyme (known for its antibacterial properties).

polyelectrolyte multilayer ; lysozyme

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