engleski

Assessement of cytotoxic and genotoxic effect of whitening toothpastes in buccal mucosal cells

Aim: To assess the cytotoxic and genotoxic effects of commercially available whitening toothpastes with different ingredients using a buccal micronucleus cytome assay. Materials and methods: This prospective, parallel randomized controlled clinical trial was conducted using a buccal micronucleus cytome assay (BMCyt assay). 198 participants were randomly divided into ten groups based on used toothpaste (9 whitening toothpastes and 1 conventional toothpaste). Buccal mucosal cell samples were taken at baseline (T0), 30 and 60 days from the beginning of treatment (T1, T2) and 30 days after using the tested toothpaste (T3). Results: Genotoxic parameters didn’t show biologically significant changes within testing whitening toothpastes. Cytotoxic parameters (number of cells with karyorrhexis and condensed chromatin) showed statistically significant increase (p<0.05) at T1 and T2 compared to baseline for three peroxide containing toothpastes. Conclusion: It can be concluded that the usage of toothpastes with whitening ingredients doesn’t lead to cytogenetic damage of buccal mucosa cells. In clinical conditions, the obtained changes can’t be considered as significant.

cytotoxicity ; genotoxicity ; buccal mucosal cells ; micronucleus assay ; whitening toothpastes

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