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"Fingerprinting" the Croatian beans - An overview of the research on Croatian common bean collection

The collection of Croatian common bean accessions, predominantly consisting of traditional cultivars (landraces), was established at the University of Zagreb Faculty of Agriculture. During the last decade it has provided a valuable material for the research on several scientific projects. The collection was first genotyped for 26 SSR markers in order to assess its genetic diversity and structure. The grouping of accessions into three major clusters corresponded well with their phaseolin type, as well as with their Mesoamerican or Andean origin. Selected set of accessions was then grown in a field trial and scored for their nutrient content. Results revealed some differences in mineral content between cultivars of a different origin, but generally mineral levels were high enough (compared to those from other authors) to consider the collection as a valuable resource for plant breeding. Further genotyping based on 6311 SNP markers provided additional confirmation of earlier results obtained using SSR markers and resolved some earlier doubts. Finally, collected data were submitted to GWAS, which resulted in discovering 22 QTNs associated with N, P, Ca, Mg and Zn content.

Traditional cultivars, genetic diversity, molecular markers, population structure, GWAS

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