NME6- an unusual nucleoside diphosphate kinase involved in basic mitochondrial functions (CROSBI ID 728985)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Herak Bosnar, Maja
engleski
NME6- an unusual nucleoside diphosphate kinase involved in basic mitochondrial functions
NME6 is a member of the nucleoside diphosphate kinase (NDPK) family of enzymes with crucial roles in controlling the cellular nucleotide homeostasis, as well as cell signaling, membrane remodeling and metastasis suppression, therefore, having a major impact on basic cellular functions and human diseases. The well characterized NME1-4 proteins are catalytically active - they transfer the terminal phosphate from d(NTP)s to d(NDP)s or proteins through a high-energy phosphohistidine intermediate. On the contrary, the data on other NME proteins, including NME6, are scarce and inconsistent. In our work we aimed to clarify and extend the overall knowledge on the human NME6 which brought us to unexpected discoveries. We have found NME6 to be present in the cell in two different isoforms. The recombinant NME6 protein is largely monomeric, and consequently, it does not exhibit the NDPK activity. Immunofluorescence and cell fractioning procedures revealed that NME6 resides in the mitochondrial matrix, probably adjacent to the mitochondrial inner membrane. Overexpression of NME6 had negative influence on oxidative phosphorylation but did not alter mitochondrial potential, mass or network characteristics. A screen for NME6 protein partners revealed its interaction with RCC1L, a protein involved in mitoribosomal assembly and translation. Therefore, our findings suggest a completely novel function for one of the NME/NDPK family members, independent of their classical phosphotransfer activity.
Mitochondria ; NME6 ; RCC1L ; Oxydative phosphorylation
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Podaci o prilogu
36-36.
2022.
objavljeno
Podaci o matičnoj publikaciji
From science to knowledge : book of abstracts
Morana, Dulić ; Nino, Sinčić ; Ivana, Vrhovac Madunić
Zagreb: Hrvatsko Društvo za Biotehnologiju
1847-7836
Podaci o skupu
Congress of the Croatian Society of Biochemistry and Molecular Biology: From Science to Knowledge (HDBMB22)
pozvano predavanje
05.07.2022-07.07.2022
Brela, Hrvatska