engleski

Regulation of Chromosome Segregation in Streptomyces coelicolor

Streptomyces is the largest genus within the phylum Actinobacteria. These filamentous gram- positive bacteria are best known as producers of various secondary metabolites, including more than two-thirds of the natural antibiotics used in medicine, biotechnology, and agriculture. Streptomyces have a very complex life cycle that begins with spore germination and the formation of vegetative mycelium from which grows areal hyphae that eventually differentiate into long chains of spores. Currently, our research group is investigating the functions, interactions, and temporal expression of genes important for DNA metabolism and chromosome segregation during the reproductive phase of Streptomyces coelicolor model organism for the Streptomyces genus. This bacterium contains a linear chromosome with a large number of functional genes (7825). Although the cell division mechanisms are similar to those found in rod‐shaped bacterium such as Escherichia coli, many aspects that control cell division and its coordination with chromosome segregation are remarkably different. We have identified paralogous single-stranded DNA-binding protein (SsbB) as one of the key players in chromosome segregation during sporulation in Streptomyces coelicolor [1]. To get a better insight into its biological role we have investigated other proteins that have been identified to contribute to chromosome organization and distribution during Streptomyces sporulation. In this study, we focused on segregation and condensation protein (ScpA). This protein is an interesting SsbB potential interactant whose role in chromosome segregation is still not well described [2]. Using different molecular methods, our goal was to investigate whether ScpA and SsbB participate in the same regulatory pathway that controls chromosome segregation. Protein-protein interaction between SsbB and ScpA was measured with circular dichroism (CD). S. coelicolor wild- type and mutant strains lacking SsbB and/or ScpA were examined using confocal microscopy. The result of our ongoing project will be presented and discussed.

Segregation ; Proteins ; Interaction

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