engleski

FIRST GLIMPSE INTO THE TOMATO AND PEPPER VIROMES OF CROATIA

Tomato is an important crop, hosting at least 312 viruses, satellites or viroids. Within the Croatian-Slovenian project “Nanopore high throughput sequencing for the resolution of problems in the plant pathogen epidemiology and diagnostics“, main sampling sites were greenhouses in the continental Croatia (Vidovec, Sedlarica). In 2021, tomatoes from Dalmatia (Turanj) and neighboring symptomatic peppers were also collected. Leaves were used for lateral flow tests (LFT) and nucleic acids extraction. For nanopore high throughput sequencing (HTS) library preparation, representative tomato DNase treated CTAB detergent extracts were pooled, rRNA depleted and polyA tailed. Based on HTS and LFT results, tomato and pepper samples were individually tested by reverse transcription polymerase chain reaction (RT-PCR) with specific primers. Preliminary results showed coinfection with potato virus S (PVS, belonging to the PVSO strain) and potato virus Y (PVY, clustering with PVY-NTN strains) in one tomato from Vidovec. PVY was also detected in other tomato samples from Vidovec and Sedlarica. A severely diseased tomato from Turanj harbored only tomato spotted wilt virus (TSWV). Peppers were either infected by cucumber mosaic virus (CMV) (two from Vidovec) or by potyviruses and a pospiviroid (one from Sedlarica and Vidovec). A pospiviroid amplicon was obtained for one of the Sedlarica PVY+ tomatoes. Partial sequences suggest Citrus exocortis viroid (CEVd) is distributed in tomatoes and peppers but this needs to be confirmed. This first glimpse into the Croatian tomato and pepper viromes suggests PVY was predominant in the continental tomatoes with a virus or a viroid occasionally in coinfections. A few peppers tested so far had viromes constituting of CMV, potyviruses and pospiviroids.

TOMATO ; PEPPER ; VIROMES ; HTS

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