engleski

LC-MS/MS as a solution in precise, selective, confirmatory and cost-effective food multi contaminant analysis

The LC-MS/MS has over the last decade matured into the leading technique for the detection of organic contaminants in Food and Feed. The sensitivity, selectivity and robustness of LC-MS/MS instruments have continuously improved, positioning them in legislation as confirmatory instruments. Although initially, it was not possible to obtain accurate results down to the ppb range (as required in legislation) without any matrix removal and analyte enrichment the latest generation of instruments can use a dilute & shoot approach. This is one of the prerequisites for multi- contaminant analysis due to the widely different physicochemical properties of all organic contaminants that are legally regulated. The scheduled MRM (multiple reaction monitoring) mode has enabled the acquisition of a large analyte lists e.g. multimycotoxins, bacterial metabolites, veterinary drug residues, and pesticide residues, where methods covering more than 500 compounds have become fairly routine1. We have applied an LC-MS/MS multicotaminant analysis method covering more than 500 fungal metabolites and a dilute & shoot approach for analysis of multiple legally regulated, mycotoxins with indicative levels and unregulated mycotoxins in cereals and feed samples from Croatia. The results showed that multiple contaminants are predominately present in analysed cereals and feed, usually grouped within one fungal genera metabolites. The 100% co-occurrence of mycotoxins with some bacterial and yeast metabolites was confirmed, and the usage of such dilute & shoot with LC-MS/MS multi-contaminant method was justified. ACKNOWLEDGEMENTS The author would like to thank the VIP Beer project for support. Josipa Golenja Would like to thank University North for support, as well as her family and friends.

mycotoxins ; LC-MS/MS ; occurence, multi-contaminant analysis, cereals

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