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Exploration of the antioxidative and anti- inflammatory effects of Aronia melanocarpa extract in an in vitro model of Parkinson’s disease

Introduction: Numerous studies have suggested that astrocytes are involved in the pathophysiology of Parkinson’s disease (PD), but most of the research on the therapeutic options in PD have been focused on the effects of potential treatments on the neurons. We have previously found that pretreatment of primary mouse astrocytes exposed to mitochondrial toxin 6-hydroxydopamine (6- OHDA) with Aronia melanocarpa (chokeberry) extract had protective effects. In this study, we aimed to explore if the protection of astrocytes in this in vitro model of PD was related to the antioxidant and anti-inflammatory effects of chokeberry. Materials and methods: Primary mouse astrocytes were grown on 6- well plates until confluent. Cells were either not treated or pretreated for 24 h with chokeberry extract (100 µg/mL), and 6- OHDA (120 μM) was added to the cell culture medium during the next 72 h at which point cell samples were prepared for the western blot analyses. Non-treated, non-injured cells were used as the control. Results: In this preliminary study, we found no significant effects of 6-OHDA application or chokeberry extract pretreatment on the expressions of antioxidant enzymes SOD-1 and GPX-1, as well as on the activation of the NFκB pathway. Conclusions: In our experimental conditions, we found no evidence on the mechanism of the observed protective effect of chokeberry extract in the used in vitro model of PD as this still needs to be the subject of further research.

Aronia melanocarpa ; astrocytes ; in vitro ; mouse ; oxidopamine ; Parkinson Disease

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