engleski

Influence of FTO Polymorphisms rs9939609, rs1421085 and rs17817449 on Metabolically (un)Healthy Obesity

OBJECTIVE Fat mass and obesity- associated(FTO)gene has been linked to obesity in whole- genome-wide studies. The aim of the study was to determine the association of the FTO gene single nucleotide polymorphism (SNPs) rs9939609, rs1421085, and rs17817449 with metabolically healthy obesity (MHO) or metabolically unhealthy obesity (MUO). METHODS 46 obese women volunteered to participate in the study (BMI ≥ 30 kg/m2). Genotyping of SNPs rs9939609, rs1421085 and rs17817449 in whole blood samples was performed by the real-time polymerase chain reaction (real-time PCR). The real- time PCR method enables the determination of allelic variants. Subject’s glucose and lipid values, blood pressure and waist circumference were measured. For the definition of metabolically healthy/unhealthy obesity we used criteria of Meigs. RESULTS All risk genotypes of the studied FTO gene polymorphisms (CC rs1421085, AA rs9939609 and GG rs17817449) were associated with higher BMI (P <0.05). In all three examined SNPs, there were 39.13% homozygotes for the risk allele. There is no statistically significant difference between MHO and MUO for these three polymorphisms. However MUO have less risk allele genotype in compare to MHO for all three polymorphisms (rs17817449 77, 42 % vs 83, 33 %, p=0, 79 ; rs1421085 77, 42 % vs 83, 33 %, p=0, 79 ; rs9939609 74, 19 % vs 91, 67 %, p=0, 96 respectively). For statistical analysis, we used Fisher-Irwin exact test. CONCLUSION These results indicate that FTO polymorphisms have no impact on metabolic status even if it is associated with obesity.An interesting finding is that patients with metabolically unhealthy obesity have less risk allele genotype whereas we expected that result for metabolically healthy obese patients. A potential explanation could be that metabolic status is under the impact of environmental factors or there could be another genetic background that we are not aware of yet. Nevertheless, future investigations that include otherFTO polymorphisms and epigenetics are required in discovering the true path of metabolic status.

FTO gene, metabolically obesity, single nucleotide polymorphism, obesity

nije evidentirano