engleski

HLA ALLELE AND HAPLOTYPE DIVERSITY IN CROATIA

Investigation of allele and haplotype distribution of genes pertaining to Human Leukocyte Antigen complex (HLA) is relevant for population studies but also in hematopoietic stem cell transplantation (HSCT) from HLA matched unrelated donor. This study encompassed 10 000 healthy unrelated volunteer donors from Croatian Bone Marrow Donor Registry (CBMDR). All subjects were analyzed for HLA-A, -B, -C and -DRB1 loci by one of the PCR based methods. A total of 49 different alleles on HLA-A locus was observed, 92 alleles on HLA-B locus, 36 alleles on HLA-C locus and 56 alleles on HLA-DRB1 locus. Most frequent allele for HLA-A locus was A*02:01 (29, 1%), for HLA-B locus most frequent was B*51:01 (10, 5%), HLA- C*07:01 (15, 8%) was the most frequent allele on HLA-C locus while for HLA-DRB1 most frequently observed allele was DRB1*03:01 (10, 6%). Most common haplotype was HLA- A*01:01C*07:01B*08:01DRB1*03:01 (4, 7%). Comparison of allele and haplotype frequencies with other populations of European anchesty showed some specificities of our population. Obtained results emphasize importance of existence of small and medium size registries of HSC donors due to their contribution to HLA allele and haplotype diversity identification. Finally, data provided by this study will serve to improve the procedure of finding the best donor for a given patient in HSCT program.

HLA ; allele ; haplotype ; Croatia

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