engleski

Biomarkers in serum and tape strips correlate with disease severity in patients with atopic dermatitis

This study investigated biomarker levels in serum and stratum corneum (SC) in different anatomical locations and their association with global and local disease severity in patients with AD. Stratum corneum (SC) samples of lesional and non-lesional skin were collected from three different anatomical sites in 25 adults with AD. Cytokine concentrations in serum and SC were measured by multiplex immunoassay and natural moisturizing levels (NMF) by liquid chromatography. In all patients, transepidermal water loss (TEWL) and pH were determined. Disease severity was assessed by global and local SCORAD. Positive correlations between serum and epidermal biomarker levels were seen for IL-18 and CTACK, whereas IL-17A and IL-31 correlated negatively. Epidermal levels of IL-5, IL-10, IL-31, IL-33, CTACK and Eotaxin were significantly higher at trunk site compared to upper- and lower extremity, with no difference in local SCORAD observed between sites. Global SCORAD correlated with lesional levels of TEWL, IL-1β and Eotaxin. Local SCORAD correlated with lesional levels of several immunoinflammatory cytokines. Clinical characteristics and biomarker levels partly explained variance in global and local SCORAD in multiple regression analyses, with no effect from anatomical collection site. Epidermal levels of TEWL, pH, TARC, CTACK, TSLP, IL-22 and IL-18 were significantly higher in lesional compared to non-lesional skin, whereas NMF levels were highest in non-lesional skin. In conclusion, serum and epidermal biomarker levels overlap to some extent, and epidermal biomarker levels show variation across anatomical sites. Global and local disease severity are associated with numerous biomarkers measured in serum and epidermis.

biomarkers, atopic dermatitis, tape st5ripping, stratum corneum

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