engleski

Microscopic appearance of the liver lesions caused by Fascioloides magna in a roe deer

To Europe Fascioloides magna is an alien trematode species which originates from the North American deer species, mainly the white-tailed deer. Roe deer (Capreolus capreolus) represents an aberrant host of F. magna in which permanent migration of juvenile flukes causes heavy destruction of the liver tissue leading to the death of the animal. Here we present histological characteristics of the area surrounding F. magna pseudocysts in roe deer liver. Liver samples were collected during the regular execution of the game management plan in the open hunting ground VII/17 - ‘’Žabjački lug – Česma’’ in Bjelovarsko-bilogorska County. Samples were stored in 10% buffered formalin and processed routinely using H&E staining. Microscopic findings were as follows. Effacing and replacing 95% of the hepatic parenchyma in the submitted section and distorting the hepatic architecture there was a fibrosing process that encompassed few trematode migration tracts. Tracts were multifocal to coalescing, variably sized (up to 4 mm in diameter) and bounded by thick and mostly mature, irregular, collagenous connective tissue (capsule) that incorporated many ova was infiltrated with moderate amounts of lymphocytes, less macrophages, plasma cells, neutrophils and eosinophils. Adjacent hepatocytes were markedly smaller (atrophic), and the lobular structure of the liver was disrupted due to abundant intersecting mature fibrous connective tissue. Mild to moderate amount of granular eosinophilic debris (necrosis) and moderate to abundant amounts of brown to black, anisotropic, granular iron-porphyrin pigment were present. Multifocally, within the fibrous tissue there were areas of increased numbers of duct profiles - ductular reaction (probably within the area of former portal tracts). Presence of elliptical, 150 x 90 um, operculated trematode eggs indicate sexual maturation of trematodes and by that prolonged survival of affected roe deer. Concludingly, the microscopic morphological diagnosis made was: hepatitis, fibrosing, multifocal to coalescing, chronic to chronic - active, with trematode migration tracts, iron-porphyrin pigment, biliary hyperplasia, and moderate numerous trematode eggs.

Fascioloides magna, roe deer, pseudocyst, aberrant host

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