engleski

Quantitative in-vivo magnetic resonance imaging of moderate perinatal hypoxia-related changes in a rat brain

AimThe in-vivo MRI study of juvenile rats aims to detect neuroradiological structural and volumetric changes consequently to a moderate perinatal hypoxic brain lesion. MethodsSixteen male Wistar Han (RccHan: WIST) rats were subjected to moderate hypoxia (8% O2, 92% N2/2h, ( 8 treated and 8 control) on the first postnatal day (P1). On P15, MRI T2-weighted scans were obtained in-vivo, and brain volume was quantified after manual delineation of regions of interest, using ITK-SNAP and custom scripts in Python 3.8. In addition, 16 post-hypoxia (8 males, 8 females) rats were sacrificed on P50, brains were instantly isolated, and immediately weighed on a digital scale. Due to the sex-related difference in size, brain mass was compared separately in males and females by student t-test. ResultsThe rats subjected to hypoxia show an indicative increase in olfactory bulb volume fraction (VF) median from the total brain volume (TBV ; 4.36+- 0.78%) and 4.55+- 0.37%, p=0, 0734). We found no difference between groups in the TBV or ventricular system VF medians. Post-hypoxic adult animals displayed a slight but significant increase in the brain mass in both males(1, 81+- 0.06 g and 1.72 +- 0.03 g, respectively, p= 0.0032) and females (1.67+- 0.05 g and 1.54+- 0.15 g, respectively, p=0.0448), in comparison to control rats. ConclusionFurther investigation is needed to understand the underlying mechanisms of demonstrated brain volume and mass changes after moderate perinatal hypoxia.

MRI ; development ; hypoxia

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