engleski

Changes in proline, total phenolic content, and antioxidant capacity related to the drought stress induced in 2 garlic landraces

Inevitably, plants are very often exposed to various abiotic stresses such as drought. Proline rapidly accumulates under abiotic stress conditions and imparts stress tolerance by maintaining osmotic balance, stabilizing proteins in membranes, and scavenging reactive oxygen species (ROS). Moreover, drought stress can reduce plant’s growth and change the levels of numerous bioactive secondary metabolites such as phenols, and thus changing the overall plant’s antioxidant capacity. The aim of this study was to test the effect of drought on level of proline, total phenolic content, and total antioxidant capacity (FRAP, DPPH, and ORAC) of 2 garlic landraces (IPT195 and IPT266). An interaction between landrace and drought stress was observed for proline level, and in IPT195 it was increased by 39% at drought treatment compared to well-watered plants in IPT195, however, no response in proline was observed for landrace IPT266. The total phenolic content and FRAP values were not affected by landrace or drought stress. A higher antioxidant capacity expressed as DPPH was observed in landrace IPT266 compared to IPT195, but it was not changed by water shortage. On the other hand, ORAC assay results show significant increase of total antioxidant capacity in landrace IPT195 after drought treatment (218%), while no significant difference was observed in landrace IPT266. According to results of proline levels and ORAC it seems that landrace IPT195 has faster response on drought stress than landrace IPT266 which may also indicate its higher tolerance to drought stress.

abiotic stress ; antioxidant capacity ; climate change ; garlic landrace ; phenolics content ; proline level ; stress tolerance ; stress response

nije evidentirano