Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

Para-N-Methylpyridinium Pyrenes: Impact of Positive Charge on ds- DNA/RNA and Protein Recognition, Photo-Induced Bioactivity, and Intracellular Localisation (CROSBI ID 316703)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Košćak, Marta ; Pehar, Isabela ; Božinović, Ksenija ; Kumar Kole, Goutam ; Sobočanec, Sandra ; Podgorski, Iva I. ; Pinterić, Marija ; Müller-Buschbaum, Klaus ; Majhen, Dragomira ; Piantanida, Ivo et al. Para-N-Methylpyridinium Pyrenes: Impact of Positive Charge on ds- DNA/RNA and Protein Recognition, Photo-Induced Bioactivity, and Intracellular Localisation // Pharmaceutics, 14 (2022), 11; 2499, 22. doi: 10.3390/pharmaceutics14112499

Podaci o odgovornosti

Košćak, Marta ; Pehar, Isabela ; Božinović, Ksenija ; Kumar Kole, Goutam ; Sobočanec, Sandra ; Podgorski, Iva I. ; Pinterić, Marija ; Müller-Buschbaum, Klaus ; Majhen, Dragomira ; Piantanida, Ivo ; Marder, Todd B.

engleski

Para-N-Methylpyridinium Pyrenes: Impact of Positive Charge on ds- DNA/RNA and Protein Recognition, Photo-Induced Bioactivity, and Intracellular Localisation

The 2- and 2, 7- substituted para-N-methylpyridinium pyrene cations show high-affinity intercalation into ds-DNAs, whereas their non- methylated analogues interacted with ds-DNA/RNA only in the protonated form (at pH 5), but not at physiological conditions (pH 7). The fluorescence from non-methylated analogues was strongly dependent on the protonation of the pyridines ; con- sequently, they act as fluorescence ratiometric probes for simultaneous detection of both ds-DNA and BSA at pH 5, relying on the ratio between intensities at 420 nm (BSA specific) and 520 nm (DNA specific), whereby exclusively ds-DNA sensing could be switched-off by adjustment to pH 7. Only methylated, permanently charged pyrenes show photoinduced cleavage of circular DNA, at- tributed to pyrene- mediated irradiation-induced production of singlet oxygen. Consequently, the moderate toxicity of these cations against human cell lines is strongly increased upon irradiation. Detailed studies revealed increased total ROS production in cells treated by the compounds studied, accompanied by cell swelling and augmentation of cellular complexity. The most photo-active 2- para-N- methylpyridinium pyrene showed significant localization at mitochondria, its photo-bioac- tivity likely due to mitochondrial DNA damage. Other derivatives were mostly non-selectively dis- tributed between various cytoplasmic organelles, thus being less photoactive.

N-methylpyridinium pyrene ; DNA sensing ; protein sensing ; singlet oxygen ; photodynamic therapy ; fluorescence ; theranostics

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

14 (11)

2022.

2499

22

objavljeno

1999-4923

10.3390/pharmaceutics14112499

Povezanost rada

Biologija, Kemija

Poveznice
Indeksiranost