engleski

Introducing green extraction technique for obtaining bioactive compounds from orange peel (Citrus sinensis L.) herbal dust

The citrus processing industry produces a huge amount of waste, which represents almost 50% of the weight of the fresh fruit. Orange peel waste (OPW) generated during citrus processing is a rich source of bioactive compounds. Orange peel powder (OPP) represents the milled OPW fraction of particle size lower than 0, 315 mm, which cannot be further used in filter tea production, but can be valorized and used as a potential source of fats, mono- and disaccharides, organic acids, polysaccharides, enzymes, flavonoids, terpenes and pigments. Conventional extractions can be applied for the isolation of these compounds but the question is whether they are profitable in terms of extraction time, required amount of solvent and efficiency. The use of supercritical carbon dioxide extraction (SFE-CO2) is widely recognized as safe and efficent solution which can be applied to replace these conventional methods. According to the green concept, SFE-CO2 is not only faster and more efficient, but also prevents the degradation of thermolabile compounds, avoiding application of toxic solvents, enabling production of solvent free extracts. Therefore, in this study, the efficiency of Soxhlet extraction (SE), as conventional method, and SFE-CO2 as advanced method for the isolation of volatile compounds from OPP was examined. During the SFE-CO2 process, the extraction time was reduced to 4 h, the temperature was kept constant (40 ºC), and the extraction pressure (100-300 bar) was varied. The obtained extracts were analyzed using GC-MS method. The dominant compounds identified were fatty acids (linoleic, hexadecanoic and oleic), 7- Methoxy-8-(2-oxo-3-methylbutyl)coumarin and osthole.

orange peel ; herbal dust ; supercritical extraction ; bioactive compounds

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