engleski

Analysis of plasma metabolome from dogs with idiopathic dilated cardiomyopathy by targeted metabolomics approach

Introduction: The second most common cause of heart failure in dogs is idiopathic dilated cardiomyopathy (iDCM), which results in heart failure or sudden cardiac death due to arrhythmia. iDCM is a primary myocardial disease characterized by systolic dysfunction and eccentric hypertrophy primarily of the left or both ventricles at the same time. The prognosis of the disease is generally unfavorable. Objectives: To discover changes in canine plasma metabolome we applied targeted metabolomics approach using liquid chromatography coupled to mass spectrometry. Methods: Plasma was collected from 8 dogs with iDCM and 12 healthy dogs in the retrospective study. Metabolite extraction were performed using the Absolute IDQ p400 kit (Biocrates) a commercially available kit for targeted metabolomics analysis of up to 408 metabolites distributed into 11 metabolite classes. Plasma samples were analyzed on the Dionex Ultimate 3000 UHPLC system (Thermo Fisher Scientific) coupled to a Q-Exactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific). The data analysis was performed according to the manufacturer’s guidelines using the Biocrates MetIDQ software. Results: Targeted metabolomic analysis resulted in a total of 199 metabolites, which were used for further statistical anaylsis. The univariate metabolomics analysis identified 14 metabolites with significantly different concentrations (p<0.05) between healthy dogs and dogs with iDCM. Amongs them, identified metabolites included amino acids, biogenic amines, acylcarnitines, triglycerides, diglycerides, and sphingomyelin. Conclusions: The research of plasma samples of dogs with iDCM discovered potential metabolites significantly changed in canine plasma metabolome. Novel identified metabolites could be potentialy used in the diagnosis and prognosis of iDCM.

metabolomics ; iDCM ; dog

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