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GC-MS METABOLOMICS INVESTIGATION OF PLASMA SAMPLES FROM DOGS WITH IDIOPATHIC DILATED CARDIOMYOPATHY

Introduction: The second most common cause of heart failure in dogs is idiopathic dilated cardiomyopathy (iDCM), which results in heart failure or sudden cardiac death due to arrhythmia. iDCM is a primary myocardial disease characterized by systolic dysfunction and eccentric hypertrophy primarily of the left or both ventricles at the same time. The prognosis of the disease is generally unfavorable. Objectives: The goal was to investigate novel metabolites and possible changes in the plasma metabolome of dogs with iDCM and healthy dogs using gas chromatography-mass spectrometry (GC- MS). Methods: Plasma of 20 dogs with iDCM were analysed in the retrospective study. Metabolite extraction were performed using the GC-MS metabolomics sample preparation protocol. In short, the plasma samples were extracted with extraction solvent, incubated, centrifuged and evaporated to dryness using a speedvac concentrator (Thermo Fisher Scientific). Instrumental analysis was carried out on a Shimadzu single quadrupole GCMS-QP2010 gas chromatograph-mass spectrometer (Shimadzu). Metabolites were identified using a commercially available GC-MS Metabolite Mass Spectral Database (Shimadzu). Results: GC-MS metabolomics analysis identified 25 metabolites in plasma samples from 20 dogs. The data processing determined that 10 of them were amino acids, 8 were organic acids, 3 were carbohydrates, 3 were fatty acids, and sugar alcohol. Statistical analysis showed that lactic acid, citric acid and alanine were more abundant in dogs with iDCM compare to the healthy dogs. Conclusions: Investigation of plasma samples from dogs with iDCM distinguishes significantly from healthy dogs. The GC-MS metabolomics revealed potential novel metabolites in the canine plasma metabolome.

GC-MS metabolomics, dogs, IDCM

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