Culturing cave mollusks in the laboratory: strategies and troubleshooting (CROSBI ID 726735)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Lukić, Marko ; Grgić, Magdalena ; Rožman, Tin ; Kuharić, Nikolina ; Jovović, Lada ; Weck, Robert ; Bilandžija, Helena
engleski
Culturing cave mollusks in the laboratory: strategies and troubleshooting
Objective obstacles such as high water levels that prevent access to caves at certain times of the year or the need to use complex tools and skills such as cave diving make biological research in caves extremely complex and occasionally dangerous. Moreover, to study physiological and behavioral adaptations of cave animals, they must be kept under controlled laboratory conditions. For this reason, we have established an animal facility in our laboratory. The available literature on invertebrate setup, operation, and care was sparse and unsuitable for the species we were studying. Fortunately, many cave biologists provided us with advice and support during the initial phase. Here we present our experiences to provide some guidelines for other researchers undertaking similar efforts. Among other animal groups, we are culturing several morphotypes of the cave snail Physella sp. and two cave and one surface species pair of bivalves: Congeria spp. and Dreissena polymorpha. We discuss good and bad practices in mollusk care - housing, water treatment, different types of food, and small aquarium design. Special attention is given to problems we have encountered with our colonies and changes we have made to address these problems, both successful and unsuccessful. We also address the general protocols required for keeping multiple species in a single facility, including procedures for decontamination of equipment and tools, and quarantine.
Dreissenids ; cave bivalve ; Physidae ; stygobiotic snails ; invertebrate laboratory colonies ; experimental animals ; animal care
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Podaci o prilogu
e87764
2022.
objavljeno
10.3897/aca.5.e87764
Podaci o matičnoj publikaciji
Proceedings of the 25th International Conference on Subterranean Biology
Moldovan, Oana T.
Pensoft Publishers
2603-3925
Podaci o skupu
25th International Conference on Subterranean Biology
poster
18.07.2022-22.07.2022
Cluj-Napoca, Rumunjska