engleski

Mapping the Domain Structure and Aggregation Propensity of Proteins Using a Gateway Plasmid Vector System

Some proteins represent members of conserved families, meaning that their domain structure can be easily predicted by comparison to homologous proteins whose structures have been solved experimentally. Many other proteins, however, do not share significant detectable homology with other proteins, often as results of high amounts of coiled-coil structure and/or intrinsically unstructured regions. These proteins include many whose aggregation is linked to human disease. Here we present a refined and reliable workflow for identifying the domains of such proteins, through cloning of multiple alternative fragments, and testing whether they form soluble, folded structures when expressed as recombinant peptides in E. coli, through the use of size exclusion chromatography. By using Gateway recombination for cloning, these fragments can then be rapidly transferred to alternate vectors for testing in mammalian cells. We then specifically illustrate its use for proteins that form pathological aggregates in disease, mapping not just their basic domain structures but also the specific subdomains responsible for aggregation

Gateway cloning ; Immunocytochemistry ; Immunofluorescent microscopy ; Protein aggregation ; Protein domains ; Protein folding ; Protein solubility ; Recombinant protein ; Size exclusion chromatography ; TRIOBP-1

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