engleski

Relative expression dynamics of GFkV, GVA and GPGV in grapevine host plants

With more than 80 viruses and virus-like agents described, grapevine is a perennial plant species seriously affected by virus diseases. Mixed infections occur naturally in grapevine host plants and contrary to the well-known grapevine viruses (leafroll and infective degeneration causal agents), limited information is presented on relative expression of grapevine fleck virus (GFkV), grapevine virus A (GVA) and grapevine pinot gris virus (GPGV). In this study, grapevine indicator plants were green grafted with 4 inoculums containing GFkV, GVA and GPGV in different combinations. Cortical scrapings were collected in 3 sampling periods: at dormancy phenological stage (5 and 16 months post inoculation) and the beginning of bud swelling (8 months post inoculation). Total RNA was extracted using commercial kit followed by cDNA synthesis. Using SYBR green based qPCR, relative expression of the virus genes was determined by normalizing their Ct values with those of housekeeping genes. This study showed that relative expression of GVA was decreasing continuously in all three sampling periods. Relative expressions of GPGV and GFkV significantly decreased in bud swelling stage compared to the first dormancy stage, but not from the second. In conclusion, relative expression of virus genes varied significantly in different sampling periods and it was virus dependent.

grapevine viruses, GVA, GFkV, GPGV, qPCR

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