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Prevalence of occult hepatitis B virus infection and characterization of HBV surface antigen mutants among individuals in Primorsko-Goranska County, Croatia over a five-year period (CROSBI ID 726671)

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Bubonja-Šonje, Marina ; Mohar Vitezić, Bojana ; Peruč, Dolores ; Peračković, Martina ; Abram, Maja Prevalence of occult hepatitis B virus infection and characterization of HBV surface antigen mutants among individuals in Primorsko-Goranska County, Croatia over a five-year period // 13. Hrvatski kongres klinićke mikrobiologije 10. Hrvatski kongres o infektivnim bolestima Šibenik, Hrvatska, 01.01.2022-01.01.2022

Podaci o odgovornosti

Bubonja-Šonje, Marina ; Mohar Vitezić, Bojana ; Peruč, Dolores ; Peračković, Martina ; Abram, Maja

engleski

Prevalence of occult hepatitis B virus infection and characterization of HBV surface antigen mutants among individuals in Primorsko-Goranska County, Croatia over a five-year period

Aim: To determine the prevalence and virological characteristics of occult hepatitis B virus (HBV) infection (OBI) in adult individuals from Primorsko-Goranska County over a five-year period. Materials and methods: Serum samples from selected anti-HBc-positive/HBsAg-negative patients (Architect, Abbott) were analyzed for the presence of HBV DNA by real-time and nested PCR. Mutational analyses of HBs-gene and polymerase gene sequences were performed using the Genetic Analyzer ABI 310 sequencer and ABI PRISM Sequencing Analysis v 5.4. software. Nucleotide sequences were aligned using MEGA software version 11. Alignment was performed using the Clustal Omega -1.2.4 program. Results: Of 24 900 sera routinely screened for HBsAg, anti-HBs and anti-HBc, 113 (0, 45%) sera were found to have an isolated anti-HBc serologic pattern. HBV DNA was detected in 12 of 113 (10, 6%) “anti-HBc only” positive sera representing 0, 048% of all routinely tested samples. Amplification of both HBV genomic segments, and sequencing results were obtained for only two samples with viremia of 50-100 IU/ml, in which subgenotype D3 were confirmed. This was expected given the extremely low viremia <6 IU/ml in the other ten HBV DNA positive samples. Mutational analyses of S-gene revealed several missense mutations. The mutations S136Y and G145R lead to a change in the polarity of aminoacids: S136Y (hydrophilic to hydrophobic) and G145R (hydrophobic to hydrophilic). Conclusion: OBI prevalence rate among the “anti- HBc only” positive population was 10, 6%. Multiple mutations of HBsAg (“S-escape” mutations) were partly responsible for its failure to be detected by a commercial serological assay (1, 77% cases). Because individuals with an isolated anti-HBc serologic pattern may have low grade chronic infection or infection with mutant strains, molecular testing is recommended on each “anti-HBc only” positive individual. The sensitivity of commercial HBsAg tests that can detect HBsAg surface mutants needs to be further improved.

occult hepatitis B virus infection, HBV surface antigen mutants

nije evidentirano

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Podaci o prilogu

nije evidentirano

nije evidentirano

Podaci o skupu

13. Hrvatski kongres klinićke mikrobiologije 10. Hrvatski kongres o infektivnim bolestima

poster

01.01.2022-01.01.2022

Šibenik, Hrvatska

Povezanost rada

Kliničke medicinske znanosti