engleski

Metabolic profiling of the Fusarium Treated and Naturally Infected Winter Wheat Seed

Wheat is one of the cereals with the highest global output. Nonetheless, agricultural losses resulting from fungal infection constitute a serious global concern and one of the most concerning plant pathogens are Fusarium spp. Mechanisms involved in resistance to infection are very complex since the processes linked to it might vary depending on the host plant. In addition, the end-use quality and technological properties of wheat flour are strongly dependent on carbohydrates, lipid and protein composition, all of which can be significantly altered by the infection. The aim of this study was to analyse the response to Fusarium infection at the metabolome level of 25 winter wheat varieties. The entire field experiment was conducted in the vegetative season 2019/2020 at location Osijek and Tovarnik, Croatia. One treatment was grown according to standard agronomical practice with no usage of fungicide, while another treatment was subjected to two inoculation events. In July of 2020, experimental plots were harvested and metabolic profiles of wheat grains were recorded using gas chromatography coupled with mass spectrometry (GC-MS). Results showed that certain amino acids varied between treatments as well as between locations. Concentrations of branched chain amino acids like valine, leucine, and isoleucine were significantly different between Osijek and Tovarnik. Two amino acids which are known to accumulate under various stresses in plants, alanine and proline, were also changed. The concentration of alanine was significantly changed between treatments, while the concentration of proline significantly differed between locations. Except for the above mentioned amino acids, the content of serine was also changed, implicating changes in energy metabolism and photorespiration following Fusarium treatment. In addition, the content of the 2-ethylhexanoic acid differed between the treatments, showing that the content of the fatty acids which play a role in plant defense and stress response was also changed. Since this research revealed more than 200 polar metabolites in control winter wheat grains and grains inoculated with Fusarium, these results provide information on how Fusarium influences those components in wheat grain. This research was funded by the European Union, who provided the EUROPEAN REGIONAL DEVELOPMENT FUND, grant number KK.01.1.1.04.0067. Financial support by the Access to Research Infrastructures activity in the Horizon2020 Programme of the EU (EPPN2020 Grant Agreement 731013) is gratefully acknowledged.

Winter Wheat ; Fusarium spp. ; Metabolic Profiling

nije evidentirano