engleski

In vitro regeneration of healthy Croatian autochthonous grapevine clones for repository clones establishment

Grapevine represents a significant crop in agricultural production in Croatia. Intensive work has been made on the Croatian autochthonous cultivars in the last two decades. A clone repository of autochthonous cultivars in virus-free conditions is safe and a legally regulated conservatory method of a specific crop's gene base. Considering different geographical origin of initial material, accessions can be multiplied in tissue culture presenting a much easier way than the classical propagation and ensuring a healthy plant material. The trial has covered 27 registered clones from 9 Croatian autochthonous cultivars. The material for in vitro establishment were 10 shoot tips from one vine of each clone. Vines are part of in situ mother blocks with the highest sanitary cleanliness. Material was taken in two periods, before and after flowering phenophase. Collected shoot tips were inoculated on a medium (Murashige and Skoog, 1 mg/l 6-benzylaminopurine + 0, 05 mg/l 3-indol-acetic acid). Regeneration of shoot tips was recorded after 6 weeks. Mature in vitro plants were subjected to acclimatization. Results achieved were in a range between 10-100% and the average percentage of regeneration is 30-40% in both periods. These preliminary research trials confirm the necessity for more detailed testing of tissue culture protocols for each cultivar of interest.

in vitro regeneration, autochthonous clones, grapevine repository, tissue culture establishment

nije evidentirano