engleski

ALKALINE AND ACID PHOSPHATASE ACTIVITY IN BLOOD PLASMA OF CHICKENS IRRADIATED BY LOW DOSE GAMMA RADIATION

In our previous paper (Kraljević et. al., 2000 ; Kraljević et al. 2002) we showed that the growth of the chickens hatched from eggs irradiated with 0.15 Gy gamma-rays before incubation was significantly higher than in controls during the fattening period (1-42 days). The concentration of total protein, glucose and cholesterol in the blood plasma of the same chickens was also significantly changed. In this paper an attempt was made to determine the effect of irradiation of eggs by low dose ionizing radiation before incubation upon activity of alkaline and acid phosphatase in the blood plasma of chickens hatched from irradiated eggs. The eggs of heavy breeding chickens were irradiated by dose of 0.15 Gy gamma radiation (60Co) before incubation. Along with the chickens which were hatched from irradiated eggs, there was a control group of chickens hatched from nonirradiated eggs. All other conditions were the same for both groups. After hatching, blood samples were taken from the wing vein on days 1, 3, 5, 7, 10, 20, 30 and 42. The activity of both enzymes was determined spectrophotometrically by using Boehringer Mannheim GmbH optimized kits. The activity of alkaline phosphatase in blood plasma was decreased on day 42, and the activity of acid phosphatase in the blood plasma of the same chickens was increased on day 42. Obtained results confirm our early obtained results that low dose of gamma radiation has effects upon metabolic processes in the chickens hatched from eggs irradiated before incubation.

low dose ionizing radiation; alkaline phosphatase; acid phosphatase; chickens

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