engleski

Quantitative Proteomics of Cerebrospinal Fluid and serum using Tandem Mass Tags in dogs with recurrent epileptic seizures

Cerebrospinal fluid (CSF) and serum are important biological materials since both can highlight potential biomarkers in canine epilepsy. This prospective study included four groups (group A: healthy dogs, groups B, C, D: dogs with epileptic seizures of different etiology). CSF and serum proteomic profiles were compared among the four groups. Samples were analyzed by a quantitative Tandem Mass Tag-based proteomic approach using LC-MS/MS. Identification and relative quantification were performed using Proteome Discoverer. Gene ontology terms were analyzed based on Canis lupus familiaris database. Eighteen (CSF) and 27 (serum) proteins displayed statistically significant altered levels among the four groups (P <0.05). CSF MMP2 and EFEMP2 appeared down- regulated whereas HP and APO-A1 were up-regulated (groups B, D). CSF CLEC3B and PEBP4 were up-regulated whereas APO-A1 was down-regulated (group C). CSF IGLL1 was down-regulated (groups B, C) and up-regulated (group D). CSF EFEMP2 was the only protein detected among the four groups and CSF PEBP4 was significantly different among the epileptic dogs (groups B, C and D). Similar to the CSF findings, serum HP and APO-A1 appeared up-regulated in all groups of epileptic dogs. CSF proteome changes could reflect that MMP2, HP and APO-A1 may contribute to a blood-brain barrier disruption (BBB) through the brain seizure-induced inflammatory process. Serum HP up-regulation indicate the passage to the CSF through the disrupted BBB. CSF MMP2 change may indicate the activation of protective mechanisms within the brain. Antiepileptic medication could influence several cellular responses and alter the CSF and serum proteome composition.

canine epilepsy, CSF, TMT, proteomics

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