engleski

Nutraceuticals from agro-waste: green extraction and innovative formulation

Agro-waste represents universally available source of biologically active compounds so strategies for its reduction should focus on development of innovative utilization approaches that would result in both, reduced ecological footprint, and development of value-added products. Our research is focused on the development of sustainable processes for extraction of functional components from olive pomace (OP) and tomato pomace (TP) and formulation of OP- and TP-based nutraceuticals with satisfactory technological and functional characteristics. Ultrasound-assisted extraction processes were developed requiring only food grade solvents, relatively short extraction times and reduced energy consumption. Formulation of dry extracts was focused on two innovative approaches: formation of inclusion complexes with cyclodextrins (CDCs) and adsorption on the surface of selenium nanoparticles using novel green synthesis protocol (forming functionalized selenium nanoparticles (fSeNPs)). CDCs and fSeNPs showed improved organoleptic/technological characteristics (particle size, bulk density, tap density, color, smell) and stability in comparison to native compounds (pure polyphenolic extracts, selenite or non-functionalized SeNPs). Innovative formulation approaches significantly improved in vitro bioavailability of Se and polyphenols in both, plants, and human models (Caco-2 cell model). CDCs and fSeNPs were shown effective as antimicrobials (antifungal activity) and antioxidants in different food-model systems (β- carotene emulsion model, meat model, oil model) indicating the possibility of application as natural antioxidants for food. Different in vitro biological models (plasimde DNA and cell models) confirmed significant antioxidant activity and cytoprotective effects of novel formulations. Obtained results shed a new light on usage of simple, efficient, and green formulation and utilization of highly potent agro-waste derived nutraceuticals.

food waste ; nutraceuticals ; antioxidants

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