Biopotential of underutilized Rosaceae inflorescences (CROSBI ID 725457)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Šola, Ivana ; Poljuha, Danijela ; Mikulic- Petkovsek, Maja ; Davosir, Dino ; Pinterić, Marija ; Bilić, Josipa ; Veberic, Josip ; Hudina, Metka ; Rusak , Gordana
hrvatski
Biopotential of underutilized Rosaceae inflorescences
The aim of this work was to assess the biopotential of the young inflorescence tissues of Prunus, Malus and Chaenomeles in order to evaluate the possibility of their application in the food industry, and to provide a polyphenolic fingerprint for their quality control. The contents of different bioactive compounds and their antioxidant capacities were spectrophotometrically measured, the main phenolic compounds were analysed using LC-DAD-MS, the antidiabetic potential was determined using α- amylase and α-glucosidase inhibition assays, the antiinflammatory potential was determined using a 5-lipoxygenase inhibition assay, and the cytotoxicity was determined by MTT assay. Using different statistical methods, the relations between the samples and the measured parameters were revealed. In total, 77 compounds were identified. The most effective in the inhibition of antidiabetic enzymes and anti-inflammatory 5- lipoxygenase was C. japonica. The inhibition of α- glucosidase was strongly positively correlated with the total and condensed tannins, procyanidin dimers and procyanidin tetramer, and was very strongly correlated with chlorogenic acid. In α-amUSAylase inhibition, C. japonica and P. serrulata ‘Kiku Shidare Zakura’ were equally efficient to the standard inhibitor, maltose. The most effective in the growth and proliferation inhibition of HepG2, HCT116 and HaCaT cells was P. avium. The results suggest Prunus, Malus and Chaenomeles inflorescences as functional food ingredients.
5-lipoxygenase ; α-amylase ; α-glucosidase ; antidiabetic potential ; anti-inflammatory potential ; cytotoxicity ; functional food ; LC-DAD-MS
nije evidentirano
engleski
Biopotential of underutilized Rosaceae inflorescences
The aim of this work was to assess the biopotential of the young inflorescence tissues of Prunus, Malus and Chaenomeles in order to evaluate the possibility of their application in the food industry, and to provide a polyphenolic fingerprint for their quality control. The contents of different bioactive compounds and their antioxidant capacities were spectrophotometrically measured, the main phenolic compounds were analysed using LC-DAD-MS, the antidiabetic potential was determined using α- amylase and α-glucosidase inhibition assays, the antiinflammatory potential was determined using a 5-lipoxygenase inhibition assay, and the cytotoxicity was determined by MTT assay. Using different statistical methods, the relations between the samples and the measured parameters were revealed. In total, 77 compounds were identified. The most effective in the inhibition of antidiabetic enzymes and anti-inflammatory 5- lipoxygenase was C. japonica. The inhibition of α- glucosidase was strongly positively correlated with the total and condensed tannins, procyanidin dimers and procyanidin tetramer, and was very strongly correlated with chlorogenic acid. In α-amUSAylase inhibition, C. japonica and P. serrulata ‘Kiku Shidare Zakura’ were equally efficient to the standard inhibitor, maltose. The most effective in the growth and proliferation inhibition of HepG2, HCT116 and HaCaT cells was P. avium. The results suggest Prunus, Malus and Chaenomeles inflorescences as functional food ingredients.
5-lipoxygenase ; α-amylase ; α-glucosidase ; antidiabetic potential ; anti-inflammatory potential ; cytotoxicity ; functional food ; LC-DAD-MS
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
283-284.
2022.
objavljeno
Podaci o matičnoj publikaciji
Zbornik sažetaka 14. hrvatskog biološkog kongresa = Book of abstracts of the 14th Croatian biological congress
Caput Mihalić, Katarina ; Mičetić Stanković, Vlatka ; Urlić, Inga ; Mešić, Armin ; Kružić, Petar
Zagreb: Hrvatsko biološko društvo
1848-5553
Podaci o skupu
14. HRVATSKI BIOLOŠKI KONGRES s međunarodnim sudjelovanjem (14th CROATIAN BIOLOGICAL CONGRESS with International Participation)
poster
12.10.2022-16.10.2022
Pula, Hrvatska