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Pregled bibliografske jedinice broj: 1223988

CRISPR/dCas9 molecular tools reveal the regulation of FUT8, MGAT4A, MGAT4B, MGAT5, MGAT3, and B4GALT1 genes by CpG methylation


Vujić, Ana; Klasić, Marija; Vičić Bočkor, Vedrana; Zoldoš, Vlatka; Pučić Baković, Maja; Lauc, Gordan
CRISPR/dCas9 molecular tools reveal the regulation of FUT8, MGAT4A, MGAT4B, MGAT5, MGAT3, and B4GALT1 genes by CpG methylation // Journal of Bioanthropology
Zagreb: Institute for Anthropological Research, 2022. str. 152-152 doi:10.54062/jb (poster, nije recenziran, sažetak, znanstveni)


CROSBI ID: 1223988 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
CRISPR/dCas9 molecular tools reveal the regulation of FUT8, MGAT4A, MGAT4B, MGAT5, MGAT3, and B4GALT1 genes by CpG methylation

Autori
Vujić, Ana ; Klasić, Marija ; Vičić Bočkor, Vedrana ; Zoldoš, Vlatka ; Pučić Baković, Maja ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Bioanthropology / - Zagreb : Institute for Anthropological Research, 2022, 152-152

ISBN
978-953-57695-4-5

Skup
The 12th ISABS Conference on Forensic and Anthropological Genetics and Mayo Clinic Lectures in Individualized Medicine

Mjesto i datum
Dubrovnik, Hrvatska, 22.06.2022. - 27.06.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
epigenome editing, gene regulation, CRISPR/dCas9, DNA methylation, protein glycosylation

Sažetak
In hepatocellular carcinoma (HCC), as well as in various other cancers, protein glycosylation is altered and as such is associated with tumor proliferation, invasion, metastasis, angiogenesis, and multidrug resistance. Mechanisms are mostly epigenetic. Indeed, aberrant DNA methylation is one of the epigenetic modifications that is highly perturbed in cancer, resulting in changes in transcriptional activity of many key genes, thus leading to characteristic cancer behavior. One group of genes, which might be affected, are glyco-genes coding for glycosyltransferases. The aim of this study is to explore epigenetic regulation of glyco-genes using cutting-edge CRISPR/Cas9 based molecular tools for epigenome editing. In hepatocellular carcinoma model cell line HepG2, we targeted seven candidate glyco-genes using dCas9-DNMT3A and dCas9-TET1 fusions, and subsequently analyzed CpG methylation, transcriptional gene activity, and whole-cell N-glycome as a final phenotype. Transfected cells were collected at two time points (8th and 12th day following transfection). Targeted methylation of selected CpG sites in ST6GAL1, FUT8, MGAT4A, MGAT4B, MGAT5, and B4GALT1 genes induced hypermethylation at these sites (up to 40% on average, depending on the gene), which was followed by a statistically significant change in transcriptional activity of all these genes except ST6GAL1. Targeted demethylation of MGAT3 gene (up to 45% on average) was accompanied by a statistically significant change in its transcription. As a final phenotype, whole-cell protein glycosylation was analyzed and changes in several glycosylation traits in HepG2 glycome were observed. These results suggest that alterations in CpG methylation lead to differential expression of glycosyltransferases, thus leading to aberrant protein glycosylation in HCC.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
EK-KF-KK.01.1.1.01.0010 - Znanstveni centar izvrsnosti za personaliziranu brigu o zdravlju (ZCIPersonHealth) (Secenji, Aleksandar, EK - KK.01.1.1.01) ( CroRIS)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb,
GENOS d.o.o.

Poveznice na cjeloviti tekst rada:

doi isabs.hr

Citiraj ovu publikaciju:

Vujić, Ana; Klasić, Marija; Vičić Bočkor, Vedrana; Zoldoš, Vlatka; Pučić Baković, Maja; Lauc, Gordan
CRISPR/dCas9 molecular tools reveal the regulation of FUT8, MGAT4A, MGAT4B, MGAT5, MGAT3, and B4GALT1 genes by CpG methylation // Journal of Bioanthropology
Zagreb: Institute for Anthropological Research, 2022. str. 152-152 doi:10.54062/jb (poster, nije recenziran, sažetak, znanstveni)
Vujić, A., Klasić, M., Vičić Bočkor, V., Zoldoš, V., Pučić Baković, M. & Lauc, G. (2022) CRISPR/dCas9 molecular tools reveal the regulation of FUT8, MGAT4A, MGAT4B, MGAT5, MGAT3, and B4GALT1 genes by CpG methylation. U: Journal of Bioanthropology doi:10.54062/jb.
@article{article, author = {Vuji\'{c}, Ana and Klasi\'{c}, Marija and Vi\v{c}i\'{c} Bo\v{c}kor, Vedrana and Zoldo\v{s}, Vlatka and Pu\v{c}i\'{c} Bakovi\'{c}, Maja and Lauc, Gordan}, year = {2022}, pages = {152-152}, DOI = {10.54062/jb}, keywords = {epigenome editing, gene regulation, CRISPR/dCas9, DNA methylation, protein glycosylation}, doi = {10.54062/jb}, isbn = {978-953-57695-4-5}, title = {CRISPR/dCas9 molecular tools reveal the regulation of FUT8, MGAT4A, MGAT4B, MGAT5, MGAT3, and B4GALT1 genes by CpG methylation}, keyword = {epigenome editing, gene regulation, CRISPR/dCas9, DNA methylation, protein glycosylation}, publisher = {Institute for Anthropological Research}, publisherplace = {Dubrovnik, Hrvatska} }
@article{article, author = {Vuji\'{c}, Ana and Klasi\'{c}, Marija and Vi\v{c}i\'{c} Bo\v{c}kor, Vedrana and Zoldo\v{s}, Vlatka and Pu\v{c}i\'{c} Bakovi\'{c}, Maja and Lauc, Gordan}, year = {2022}, pages = {152-152}, DOI = {10.54062/jb}, keywords = {epigenome editing, gene regulation, CRISPR/dCas9, DNA methylation, protein glycosylation}, doi = {10.54062/jb}, isbn = {978-953-57695-4-5}, title = {CRISPR/dCas9 molecular tools reveal the regulation of FUT8, MGAT4A, MGAT4B, MGAT5, MGAT3, and B4GALT1 genes by CpG methylation}, keyword = {epigenome editing, gene regulation, CRISPR/dCas9, DNA methylation, protein glycosylation}, publisher = {Institute for Anthropological Research}, publisherplace = {Dubrovnik, Hrvatska} }

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