Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 1223922

Rotavirus A infection in hospitalized children in Croatia during three seasons: the predominance of G3 genotype and the emergence of intergenogroup reassortant strains


Brnić, Dragan; Kunić, Valentina; Mikuletič, Tina; Steyer, Andrej; Kogoj, Rok; Kovačević, Alen; Krešić, Nina; Konjik, Vlatka; Sipl, Mirna; Roksandić Križan, Ivana et al.
Rotavirus A infection in hospitalized children in Croatia during three seasons: the predominance of G3 genotype and the emergence of intergenogroup reassortant strains // 14th International dsRNA Virus Symposium
Banff, Kanada, 2022. (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 1223922 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Rotavirus A infection in hospitalized children in Croatia during three seasons: the predominance of G3 genotype and the emergence of intergenogroup reassortant strains

Autori
Brnić, Dragan ; Kunić, Valentina ; Mikuletič, Tina ; Steyer, Andrej ; Kogoj, Rok ; Kovačević, Alen ; Krešić, Nina ; Konjik, Vlatka ; Sipl, Mirna ; Roksandić Križan, Ivana ; Jukić Guć, Jelena ; Krželj, Vjekoslav ; Šoprek, Silvija ; Tešović, Goran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
14th International dsRNA Virus Symposium

Mjesto i datum
Banff, Kanada, 10.10.2022. - 14.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Rotavirus A, reassortment, molecular epidemiology, genetic diversity, NGS, Croatia, humans

Sažetak
Rotavirus A (RVA), in countries like Croatia with low vaccination coverage, represents an important healthcare burden due to frequent hospitalizations. The aim of the study was to investigate rotavirus diversity in hospitalized children in Croatia and to evaluate the potential zoonotic background of circulating strains. During three consecutive RVA seasons (2018-2021), we have collected 602 RVA positive samples from children admitted to three regional hospitals. Multiplex semi-nested RT-PCR and Sanger sequencing were applied for VP7/VP4 genotyping. For G1 and G3 strains, NSP5 and VP6 segments were additionally genotyped and selected strains were further analyzed by NGS on Illumina platform. Overall, we detected eight and five different G and P genotypes, respectively. In descending order, G3, G9, G2, G1, G4, G6, G8, G10 and P[8], P[4], P[9], P[6], P[14]. The G3 genotype was predominant (54%) with rising season-to-season prevalence of G3 equine-like (G3e) lineage. Furthermore, P[8] genotype was detected in 79% of samples. Interestingly, a high share (15%) of mixed G genotypes was observed. On the contrary, strains with zoonotic background were infrequent (1.6%). The NSP5/VP6 genotyping and NGS confirmed the emergence of intergenogroup reassortant strains ; G1P[8] and G3eP[8], both in DS-1-like and Wa-like constellations. Notable genetic heterogeneity and sporadic detection of RVA strains with zoonotic background were reported in hospitalized children in Croatia. A significant finding is the circulation of intergenogroup reassortant strains. These results provide a valuable source of knowledge prerequisite in considering potential introduction of vaccination in the national immunization program.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina



POVEZANOST RADA


Projekti:
UIP-2017-05-8580 - Rotavirusi u ekosustavu Republike Hrvatske: molekularna epidemiologija i zoonotski potencijal (REco) (Brnić, Dragan, HRZZ - 2017-05) ( CroRIS)

Ustanove:
Hrvatski veterinarski institut, Zagreb,
Klinički bolnički centar Osijek,
KBC Split,
Klinika za infektivne bolesti "Dr Fran Mihaljević",
Sveučilište u Splitu Sveučilišni odjel zdravstvenih studija


Citiraj ovu publikaciju:

Brnić, Dragan; Kunić, Valentina; Mikuletič, Tina; Steyer, Andrej; Kogoj, Rok; Kovačević, Alen; Krešić, Nina; Konjik, Vlatka; Sipl, Mirna; Roksandić Križan, Ivana et al.
Rotavirus A infection in hospitalized children in Croatia during three seasons: the predominance of G3 genotype and the emergence of intergenogroup reassortant strains // 14th International dsRNA Virus Symposium
Banff, Kanada, 2022. (poster, međunarodna recenzija, sažetak, znanstveni)
Brnić, D., Kunić, V., Mikuletič, T., Steyer, A., Kogoj, R., Kovačević, A., Krešić, N., Konjik, V., Sipl, M. & Roksandić Križan, I. (2022) Rotavirus A infection in hospitalized children in Croatia during three seasons: the predominance of G3 genotype and the emergence of intergenogroup reassortant strains. U: 14th International dsRNA Virus Symposium.
@article{article, author = {Brni\'{c}, Dragan and Kuni\'{c}, Valentina and Mikuleti\v{c}, Tina and Steyer, Andrej and Kogoj, Rok and Kova\v{c}evi\'{c}, Alen and Kre\v{s}i\'{c}, Nina and Konjik, Vlatka and Sipl, Mirna and Roksandi\'{c} Kri\v{z}an, Ivana and Juki\'{c} Gu\'{c}, Jelena and Kr\v{z}elj, Vjekoslav and \v{S}oprek, Silvija and Te\v{s}ovi\'{c}, Goran}, year = {2022}, chapter = {2}, keywords = {Rotavirus A, reassortment, molecular epidemiology, genetic diversity, NGS, Croatia, humans}, title = {Rotavirus A infection in hospitalized children in Croatia during three seasons: the predominance of G3 genotype and the emergence of intergenogroup reassortant strains}, keyword = {Rotavirus A, reassortment, molecular epidemiology, genetic diversity, NGS, Croatia, humans}, publisherplace = {Banff, Kanada}, chapternumber = {2} }
@article{article, author = {Brni\'{c}, Dragan and Kuni\'{c}, Valentina and Mikuleti\v{c}, Tina and Steyer, Andrej and Kogoj, Rok and Kova\v{c}evi\'{c}, Alen and Kre\v{s}i\'{c}, Nina and Konjik, Vlatka and Sipl, Mirna and Roksandi\'{c} Kri\v{z}an, Ivana and Juki\'{c} Gu\'{c}, Jelena and Kr\v{z}elj, Vjekoslav and \v{S}oprek, Silvija and Te\v{s}ovi\'{c}, Goran}, year = {2022}, chapter = {2}, keywords = {Rotavirus A, reassortment, molecular epidemiology, genetic diversity, NGS, Croatia, humans}, title = {Rotavirus A infection in hospitalized children in Croatia during three seasons: the predominance of G3 genotype and the emergence of intergenogroup reassortant strains}, keyword = {Rotavirus A, reassortment, molecular epidemiology, genetic diversity, NGS, Croatia, humans}, publisherplace = {Banff, Kanada}, chapternumber = {2} }




Contrast
Increase Font
Decrease Font
Dyslexic Font