engleski

Liquid-liquid extraction of endo-1,4-xylanase in a microextractor

Nowadays, there’s a growing interest in environmentally friendly extractions and many efforts were made to develop clean, sustainable, and efficient extraction processes. Aqueous twophase systems (ATPSs) are considered an effective, versatile and important new green technology for downstream processing of biomolecules. ATPSs are liquid-liquid systems that can be obtained by mixing certain polymers, ionic liquids, deep eutectic solvents (DES) and short- chain alcohols with salts or detergents. In this work, the extraction of the enzyme endo-1, 4-β- xylanase from Trametes versicolor was performed in a microextractor with two ATPSs. The first ATPS was formed by mixing DES betaine:urea (1:3) with 30% (w/w) water and K2HPO4 and the second was formed as a mixture of polyethylene glycol (PEG1540) and C6H9Na3O9. Extraction with ATPS based on PEG1540 was performed under previously determined optimal process conditions (γenyzme = 0.3 mg/mL, wPEG = 0.21). Extraction efficiency of E = 100%±1.20% was achieved for residence time of τ = 0.25 min. In addition, continuous extraction was performed in a microextractor with crude xylanase and extraction efficiency of E = 58.99% ±5.69% was achieved. When extraction was performed using DES, the highest extraction efficiency was only E = 16.29%±3, 28% making this system unfavorable compared to ATPS based on PEG.

ATPS ; xylanase ; liquid-liquid extraction ; microextractor

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