engleski

Incidence, partal molecular characterization and transmission modes of grapevine virus G

Grapevine virus G (GVG) is one of eight vitiviruses discovered in grapevines in the last decade. GVG was detected in Croatia in 2018, and current research indicates its widespread in several wine-growing regions of the world. The aim of this study was to determine the incidence of GVG in Croatia, variability of the part of the coat protein gene (CP), as well as the modes of transmission and possible alternative hosts. For this purpose, 4302 samples of grapevine petioles were collected in the collection plantations and commercial vineyards and the RNA was extracted. PCR detection protocols (conventional and realtime) were developed using the available virus genome data. Four different methods in transmission trials were used: vine mealybug Planoccocus ficus, mechanical transmission, transmission by seeds and grafting. In the Croatian continental part, the virus was confi rmed in the collection plantation “Jazbina” and the collection of viruses (Faculty of Agriculture, Zagreb) in percentages of 7.1% and 10.7%, respectively, but not in samples from 16 tested commercial vineyards. In the coastal part, GVG was detected in the collection plantation located in Split (4.8%) and in 22 out of 72 (30, 6%) commercial vineyards tested with a total occurrence rate of 10.6%. CP region of Croatian GVG isolates showed location dependent heterogeneity. Vine to vine transmission tests using vine mealybug showed 15% efficiency. Also, transmission by grafting has been confi rmed, while other types of transmission, as well as alternative hosts, have not been confirmed.

RT-PCR, qPCR, sequencing, phylogenetics, Planococcus ficus

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