engleski

IN VIVO GROWTH INHIBITION AND ANTI-ANGIOGENIC EFFECTS OF RESVERATROL AND ITS NANOCRYSTALS ON SOLID TUMOR IN MICE

Due to low toxicity and multiple mechanisms, natural compounds such as resveratrol could play a significant role in the treatment of cancers. Nevertheless, therapeutic use of resveratrol is limited by its physico-chemical and pharmacokinetic properties. To overcome its traits, we produced rasveratrol as nanocrystals suspension, as several formulations of flavonoid nanocrystals have shown enhanced pharmacological effects. The aim of this study was to investigate in vivo effects resveratrol and its nanocrystals on angiogenesis-dependent, fast-growing Ehrlich ascites tumor (EAT) in solid form, as well as potential toxicity caused by the interacion of nanocrystals with biological tissue through biochemical and histological changes of kidney and liver. Tumor was induced by subcutaneous injection of 1 × 106 EAT cells into the muscle tissue of the right hind leg of Swiss albino mice. Treatment of animals with EAT started on day 1 of the experiment by intratumoral injection of resveratrol or resveratrol nanocrystals at dose of 50 mg/kg every other day for 14 days. The results indicate that resveratrol and resveratrol nanocrystals have an inhibitory effect on tumor growth. Reduced microvessel density in the tumor, liver and kidney tissue confirms the anti-angiogenic potential of resveratrol in vivo. Antioxdant and anti- inflammatory effect is seen through reduced NO levels and elevated Arg1 levels in kidney tissue, as well as biochemical parameters in serum. Based on the obtained results, we can conclude that resveratrol and its nanocrystals successfully inhibit the growth and angiogenesis of solid form of EAT and have protective effect on the liver and kidney.

resveratrol, nanocrystals, solid tumor model

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