engleski

Antimicrobial susceptibility and multilocus sequence typing of Campylobacter jejuni isolates from dogs with diarrhea

Campylobacteriosis is one of the most common zoonoses worldwide, with dogs being one of the possible sources of infection. The aim of this study was to investigate the antimicrobial susceptibility and sequence types of Campylobacter jejuni isolated from the feces of dogs with diarrhea. Campylobacter spp. were isolated on modified charcoal-cefoperazone-deoxycholate agar (mCCDA). Species was identified by MALDI TOF MS. Antimicrobial susceptibility of isolates was 4th International Conference of the European College of Veterinary Microbiology, 15-17 September 2022, Bari, Italy 50 tested using the disk diffusion method according to the EUCAST. Multi-locus sequence typing (MLST) was performed according to Dingle et al (2001). From 248 dogs, 46 (18.5%) Campylobacter spp. were isolated. Of these, 26 (56.5%) were C. jejuni. Resistance to ciprofloxacin was 57.7%, to tetracycline 23.1%, and to erythromycin 3.8%. Five (19.2%) isolates were resistant to two antimicrobials ; four to ciprofloxacin and tetracycline and one to ciprofloxacin and erythromycin. Fifteen isolates were randomly selected for MLST and classified into six clonal complexes (CC-21, CC-206, CC-403, CC-45, CC-443, and CC-48) and 10 sequence types ; three each from ST-1943 and ST-10039, two from ST-538, and one each from ST3156, ST-19, ST-2086, ST-572, ST-3335, ST-475, and ST-51. Six of these STs have not been previously detected in dogs. Six STs have not been confirmed in Croatia to date. High genetic diversity was found among the typed isolates. Given the increasing contact of dogs and humans, continuous epidemiological studies of campylobacteriosis and antimicrobial susceptibility of isolates from all sources are needed. Acknowledgement The participation in the “4th ICECVM Conference 2022” is supported by COST 18217 European Network for Optimization of Veterinary Antimicrobial Treatment.

Campylobacter ; Dog ; Antimicrobial resistance

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