engleski

Antioxidative activity of selenium nanoparticles functionalized with polyphenols from olive pomace extract

Introduction: Selenium nanoparticles (SeNPs) have enhanced biocompatibility compared to inorganic and organic selenium forms. Surface of nanoparticles (NPs) can be functionalized to further improve their physio-chemical characteristics and biological activity (1). In this work, polyphenols from olive pomace were utilized to synthesize SeNPs with improved antioxidative activity. Materials and methods: Polyphenolic olive pomace extract (OPE) was prepared according to Albahari et. al (2) and used for surface functionalization of SeNPs. The NPs' size and zeta potential were determined using dynamic light scattering and electrophoretic light scattering techniques. To test the antioxidative activity of SeNPs, HepG2 cells were treated with SeNPs, followed by addition of prooxidant tert- butyl hydroperoxide. The relative amount of reactive oxygen species (ROS) was then measured using DCFH-DA dye. Results: Synthesis resulted in different types of nanoparticles: polyvinylpyrrolidone stabilized (PVPSeNPs), polysorbate stabilized (PSSeNPs), polyvinylpyrrolidone stabilized and functionalized with OPE (fPVPSeNPs) and polysorbate stabilized and functionalized with OPE (fPSSeNPs). The size of NPs ranged from 56.0 nm to 181.7 nm and zeta potential from -5.9 to -33.0 mV. The antioxidative activity was shown for fPVPSeNPs and fPSSeNPs in tested concentrations. Conclusion: Functionalization of SeNPs with OPE resulted in improved stabilization and activity against ROS induction in HepG2 cells.

selenium nanoparticles ; polyphenols ; in vitro ; antioxidative activity

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