engleski

Dietary intake of n-3 PUFAs enriched hen eggs changes Treg population frequencies in PBMCs of young, healthy individuals –randomized controlled study

Objectives: To determine the effects of n-3 polyunsaturated fatty acid (PUFA) supplementation, i.e., α-linolenic (~230 mg), eicosapentaenoic (~15 mg), and docosahexaenoic acid (~105 mg), on the prevalence of T regulatory (Treg) lymphocytes and their subsets in healthy individuals. Participants and Methods: 40 participants were randomized and divided into: 1) Control group (N=21) that consumed regular hen eggs (n-3 PUFAs: ~249 mg/per day), 2) n-3 PUFAs group (N=19) that consumed enriched hen eggs (n-3 PUFAs: ~1053 mg/per day). The study protocol included two appointments and participants were instructed to eat three hard- boiled hen eggs/day for three weeks. Venous blood samples were taken before and after the dietary protocol for serum and peripheral blood mononuclear cells isolation. Frequency of Treg lymphocytes were determined by flow cytometry. Measurements were carried out by BD FACSCanto II cytometer equipped with blue Argon 488 nm and Red HeNe 633 nm laser lines. Results: Both study groups showed reduced frequencies of peripheral Treg lymphocytes. There was a significant reduction of CD4+CD25+Foxp3high subpopulation corresponding to ‘real’ regulatory T cells (nTreg) in both groups. Further, rates of recently activated T cells were positively associated, while the % of nTreg was inversely related to serum IL-22 levels in the n-3 PUFAs group. Conclusion: Both dietary protocols resulted in reduced frequencies of peripheral nTreg lymphocytes. Although, regarding the same study groups, we previously reported that functional capacity of T cells for cytokine secretion was significantly altered only in the n-3 PUFAs group through increased anti-inflammatory and reduced pro-inflammatory cytokine secretion.

inflammation, functional food, Treg lymphocytes, cytokines

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