hrvatski

Određivanje zemljopisnog podrijetla hrvatskog meda pomoću stabilnih izotopa, mineralnih tvari i kemometrije

Honey is valuable bee product mainly composed of carbohydrates (fructose and glucose, 65-75%) and water (15-20%), while other components which are present in small amounts are responsible for sensory and nutritive honey characteristics. For many years honey is recognised as a high-quality food which is why it is often subject of adulteration (addition of sugar syrups, mislabelling of botanical and geographical origin). Considerable efforts are being made to find new analytical methods for assessing honey authenticity. One of the newer analytical approaches in determination of botanical and geographical origin of honey is usage the combination of stable isotopes and mineral composition together with chemometrics. The aim of this work was to examine weather is possible to distinguish honey samples of the same botanical origin collected in two different geographical regions of Croatia (continental and coastal) by using stable carbon (δ13C) and nitrogen (δ15N) isotope ratio and mineral composition. Lime (Tilia spp.) and black locust (Robinia pseudoacacia L.) honey samples were collected in beekeeping season 2014 and 2015, respectively. Stable isotopes (δ13C and δ15N) in honey and isolated honey proteins were determined by elemental analyser isotope ratio mass spectrometer (EA-IRMS). Mineral elements (Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Na, Ni, P, Pb, S, Zn) were determined by inductively coupled plasma - optical emission spectrometry (ICP-OES) with previous microwave digestion of the samples. Results of the PCA analysis showed that 87% of black locust honey samples from two regions were distinguished by using only IRMS parameters, while in combination with ICP parameters classification success was 100%. Lime honey samples were successfully classified according to region of origin by using only IRMS parameters. Combination of IRMS and ICP parameters together with PCA analyses could be useful tool in assessing honey geographical origin.

honey ; geographical origin ; stable isotopes ; mineral composition ; PCA

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