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Physiological and biochemical response of Arabidopsis thaliana (L.) Heynh. with modified BPM expression to UV-B radiation

Solar radiation is a source of energy for photosynthesis and an important signal for regulating plant growth and development. However, high light intensity and ultraviolet (UV) radiation can lead to oxidative stress in plants. The main objective of this research was to evaluate the effect of UV‑B radiation on the model plant Arabidopsis thaliana. Photosynthetic efficiency, hydrogen peroxide (H2O2) content, level of lipid peroxidation, antioxidant enzyme activity and proline content were used as measurable parameters to evaluate the effect of UV‑B stress on plants. In addition to the wild type, the A. thaliana lines with altered expression of BPM genes (oeBPM1 and amiR-bpm) were studied. BPM proteins belong to the MATH‑BTB (BPM) protein family, which is involved in the regulation of developmental processes and stress response in plants. Immunodetection of the BPM1‑GFP protein in the oeBPM1 line confirmed the highest accumulation of the protein after treatment with UV‑B radiation dose of ~800 J cm-2 . After exposure to UV-B, changes were observed in the H2O2 content of the wild type and the oeBPM1 line, as well as in the antioxidant enzyme activities of the wild type, and in the maximum quantum efficiency of photosystem II in the oeBPM1 line. The oeBPM1 line showed a better stress response than the other two lines due to a stronger basic activity of antioxidant enzymes. The amiR-bpm line showed a higher H2O2 content and a higher level of lipid peroxidation compared to the wild type and the oeBPM1 line. The obtained results suggest that BPM proteins are involved in the regulation of certain physiological and biochemical processes under both optimal and UV‑B stress conditions.

antioxidant enzymes ; H2O2 ; lipid peroxidation ; MATH‑BTB ; photosynthetic efficiency

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