engleski

Influence of bioclimatic conditions on the pyrethrin profiles of Dalmatian pyrethrum natural populations

Pyrethrin is a leading plant biopesticide that is highly effective against numerous pests and has little or no negative impact on human health and the environment. It is a specialized metabolite from Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevis.) Sch. Bip. ; Asteraceae), a plant species native to the eastern Adriatic coast. Pyrethrin consists of six compounds, pyrethrin I and II, cinerin I and II, and jasmolin I and II, with pyrethrin I and II accounting for the largest proportion of total pyrethrins. In order to determine the intraspecific biochemical variability and to investigate the influence of different ecological conditions on the accumulation of the individual compounds, 15 natural Dalmatian pyrethrum samples were collected. Ultrasound-assisted extraction was used for the pyrethrin extraction, followed by their qualitative and quantitative analysis on HPLC-UV- DAD. Total pyrethrin content ranged from 0.82 % to 1.27% of flower dry weight (DW). Populations were clustered in five bioclimatic groups ; A (Northern Adriatic populations and one coastal population from central Adriatic), B (two central Adriatic island populations), C (two central Adriatic island populations), D (Southern Adriatic island populations), E (inland Southern Adriatic populations, populations from Bosnia and Herzegovina and Montenegro), each characterized by a unique chemical profile. The highest average values for pyrethrin I (53.07% of total pyrethrin), total pyrethrin content (1.06 % DW) and the ratio of pyrethrin I and II (1.85) were found in the bioclimatic group E. The observed variability was partially explained by significant correlation found between the levels of some pyrethrin compounds and bioclimatic variables (e.g., BIO03 Isothermality and BIO04 Temperature seasonality). The results will support the selection of populations for breeding programs aimed at producing cultivars with desirable biochemical properties and adaptation to different bioclimatic conditions.

Biopesticides ; biochemical variability ; pyrethrin ; Tanacetum cinerariifolium

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