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New insight into the role of PTCH1 protein in serous ovarian carcinomas (CROSBI ID 314864)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Karin-Kujundžić, Valentina ; Covarrubias‑Pinto, Adriana ; Škrtić, Anita ; Vranić, Semir ; Šerman, Ljiljana New insight into the role of PTCH1 protein in serous ovarian carcinomas // International journal of oncology, 61 (2022), 6; 145, 11. doi: 10.3892/ijo.2022.5435

Podaci o odgovornosti

Karin-Kujundžić, Valentina ; Covarrubias‑Pinto, Adriana ; Škrtić, Anita ; Vranić, Semir ; Šerman, Ljiljana

engleski

New insight into the role of PTCH1 protein in serous ovarian carcinomas

The Hedgehog (Hh) signaling pathway is essential for normal embryonic development, while its hyperactivation in the adult organism is associated with the development of various cancers. The role of the Hh signaling pathway in ovarian cancer has not been sufficiently investigated. Therefore, the present study investigated the role of protein patched homolog 1 (PTCH1), a component of the Hh signaling pathway, and changes in the promoter methylation status of the corresponding gene in a cohort of low‑(LGSC) and high‑grade serous ovarian carcinomas (HGSC) and HGSC cell lines (OVCAR8 and OVSAHO). PTCH1 protein expression level was analyzed using immunohistochemistry in tissue samples and immunofluorescence and western blotting in cell lines. DNA methylation patterns of the PTCH1 gene were analyzed using methylation‑specific PCR. PTCH1 protein expression was significantly higher in HGSCs and LGSCs compared with controls (healthy ovaries and fallopian tubes). Similarly, ovarian cancer cell lines exhibited significantly higher PTCH1 protein expression compared with a normal fallopian tube non‑ciliated epithelial cell line (FNE1). PTCH1 protein fragments of different molecular weights were detected in all cell lines, indicating possible proteolytic cleavage of this protein, resulting in the generation of soluble N‑terminal fragments that are translocated to the nucleus. DNA methylation of the PTCH1 gene promoter was exclusively detected in a proportion of HGSC (13.5%) but did not correlate with protein expression. PTCH1 protein was highly expressed in serous ovarian carcinoma tissues and cell lines, while PTCH1 promoter methylation was only detected in HGSC. Further investigation is required to elucidate the possible mechanisms of PTCH1 activation in serous ovarian carcinomas.

ovarian cancer ; serous ovarian carcinoma ; ovarian cancer cell lines ; Hedgehog signaling pathway ; protein patched homolog 1

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nije evidentirano

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nije evidentirano

Podaci o izdanju

61 (6)

2022.

145

11

objavljeno

1019-6439

1791-2423

10.3892/ijo.2022.5435

Povezanost rada

Biologija, Temeljne medicinske znanosti

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