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Pregled bibliografske jedinice broj: 1220460

The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin


Trupinić, Monika; Kokanović, Barbara; Ponjavić, Ivana; Barišić, Ivan; Šegvić, Siniša; Ivec, Arian; Tolić, Iva M.
The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin // Current Biology, 32 (2022), 11; 2480-2493e6 doi:10.1016/j.cub.2022.04.035 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 1220460 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin

Autori
Trupinić, Monika ; Kokanović, Barbara ; Ponjavić, Ivana ; Barišić, Ivan ; Šegvić, Siniša ; Ivec, Arian ; Tolić, Iva M.

Izvornik
Current Biology (0960-9822) 32 (2022), 11; 2480-2493e6

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Mitotic spindle ; Mitosis ; Chirality ; Twist ; Torques ; Rotation ; Motor proteins ; Kinesins ; Augmin ; Spindle compression

Sažetak
Forces produced by motor proteins and microtubule dynamics within the mitotic spindle are crucial for proper chromosome segregation. In addition to linear forces, rotational forces or torques are present in the spindle, which are reflected in the left-handed twisted shapes of microtubule bundles that make the spindle chiral. However, the biological role and molecular origins of spindle chirality are unknown. By developing methods for measuring the spindle twist, we show that spindles are most chiral near the metaphase-to-anaphase transition. To assess the role of chirality in spindle mechanics, we compressed the spindles along their axis. This resulted in a stronger left-handed twist, suggesting that the twisted shape allows for a mechanical response to forces. Inhibition or depletion of motor proteins that perform chiral stepping, Eg5/kinesin-5, Kif18A/kinesin-8, MKLP1/kinesin-6, and dynein, decreased the left-handed twist or led to right- handed twist, implying that these motors regulate the twist by rotating microtubules within their antiparallel overlaps or at the spindle pole. A right-handed twist was also observed after the depletion of the microtubule nucleator augmin, indicating its contribution to the twist through the nucleation of antiparallel bridging microtubules. The uncovered switch from left- handed to right-handed twist reveals the existence of competing mechanisms that promote twisting in opposite directions. As round spindles are more twisted than the elongated ones are, we infer that bending and twisting moments are generated by similar molecular mechanisms and propose a physiological role for spindle chirality in allowing the spindle to absorb mechanical load.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti



POVEZANOST RADA


Projekti:
EK-647077 - Nova klasa mikrotubula u vretenu koja djeluje silama na kinetohore (NewSpindleForce) (Tolić, Iva, EK ) ( CroRIS)
HRZZ-IP-2019-04-5967 - Ravnoteža sila i momenta sila u diobenom vretenu (SpindleForceBalance) (Pavin, Nenad, HRZZ - 2019-04) ( CroRIS)
EK-EFRR-KK.01.1.1.04.0057 - Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini (IPSted) (Tolić, Iva Marija, EK - KK.01.1.1.04) ( CroRIS)
HRZZ-PZS-2019-02-7653 - Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena (SpindleForm) (Tolić, Iva, HRZZ - 2019-02) ( CroRIS)
EK-855158 - Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima (ANEUPLOIDY) (Tolić, Iva; Pavin, Nenad, EK ) ( CroRIS)

Ustanove:
Fakultet elektrotehnike i računarstva, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Trupinić, Monika; Kokanović, Barbara; Ponjavić, Ivana; Barišić, Ivan; Šegvić, Siniša; Ivec, Arian; Tolić, Iva M.
The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin // Current Biology, 32 (2022), 11; 2480-2493e6 doi:10.1016/j.cub.2022.04.035 (međunarodna recenzija, članak, znanstveni)
Trupinić, M., Kokanović, B., Ponjavić, I., Barišić, I., Šegvić, S., Ivec, A. & Tolić, I. (2022) The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin. Current Biology, 32 (11), 2480-2493e6 doi:10.1016/j.cub.2022.04.035.
@article{article, author = {Trupini\'{c}, Monika and Kokanovi\'{c}, Barbara and Ponjavi\'{c}, Ivana and Bari\v{s}i\'{c}, Ivan and \v{S}egvi\'{c}, Sini\v{s}a and Ivec, Arian and Toli\'{c}, Iva M.}, year = {2022}, pages = {2480-2493e6}, DOI = {10.1016/j.cub.2022.04.035}, keywords = {Mitotic spindle, Mitosis, Chirality, Twist, Torques, Rotation, Motor proteins, Kinesins, Augmin, Spindle compression}, journal = {Current Biology}, doi = {10.1016/j.cub.2022.04.035}, volume = {32}, number = {11}, issn = {0960-9822}, title = {The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin}, keyword = {Mitotic spindle, Mitosis, Chirality, Twist, Torques, Rotation, Motor proteins, Kinesins, Augmin, Spindle compression} }
@article{article, author = {Trupini\'{c}, Monika and Kokanovi\'{c}, Barbara and Ponjavi\'{c}, Ivana and Bari\v{s}i\'{c}, Ivan and \v{S}egvi\'{c}, Sini\v{s}a and Ivec, Arian and Toli\'{c}, Iva M.}, year = {2022}, pages = {2480-2493e6}, DOI = {10.1016/j.cub.2022.04.035}, keywords = {Mitotic spindle, Mitosis, Chirality, Twist, Torques, Rotation, Motor proteins, Kinesins, Augmin, Spindle compression}, journal = {Current Biology}, doi = {10.1016/j.cub.2022.04.035}, volume = {32}, number = {11}, issn = {0960-9822}, title = {The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin}, keyword = {Mitotic spindle, Mitosis, Chirality, Twist, Torques, Rotation, Motor proteins, Kinesins, Augmin, Spindle compression} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE
  • Nature Index


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