engleski

Preliminary study results on the gene expression analysis of intramuscular fat content in Black Slavonian pig

Intramuscular fat (IMF) plays an important role in determination of meat quality and directly contributes to the positive technological and sensory characteristics such as taste, texture and juiciness. In Black Slavonian pig (BS), numerous studies reported much higher IMF content compared to other local breeds, therefore, understanding the genetic mechanisms of IMF in BS becomes one of the main challenges in scientific research. Transcriptome and gene expression analyses using RNA-seq technology is approach which enables the identification of genes that are differentially expressed in distinct phenotypes. In the last few years, the majority of gene expression studies has been carried out on modern pig breeds, therefore such analyses on local breeds would significantly contribute to the pig breeding of local breeds, but also to the commercial breeding programs. For the analysis of gene expression for IMF in this study, out of the total of 80 pigs in full-sib design, 10 sib pairs with extremely high and low IMF phenotypes were selected, and also analyzed for performance and meat quality traits. Whole transcriptome analysis was performed using RNA-seq technology, while candidate genes were further verified using quantitative real-time PCR (qPCR) analysis. The results from this study will certainly contribute to the understanding of genetic mechanisms of IMF in BS, and will also provide new possibilities for improvement of breeding program.

Gene expression ; intramuscular fat content ; Black Slavonian pig ; genomics

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