THE ROLE AND DIVERSITY OF PIR PROTEINS IN CELL WALLS OF BUDDING YEASTS (CROSBI ID 724140)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Žunar, Bojan ; Lozančić, Mateja ; Martinić-Cezar, Tea ; Teparić, Renata ; Mrša, Vladimir
engleski
THE ROLE AND DIVERSITY OF PIR PROTEINS IN CELL WALLS OF BUDDING YEASTS
Pir proteins (proteins with internal repeats) are the most prominent proteins that yeast Saccharomyces cerevisiae covalently binds to its cell wall through β-1, 3-glucan residues. As such, they are regularly used for yeast surface display when the displayed protein needs to be bound close to its N-termini. However, the role of Pir proteins in cells remains unclear, and their diversity in closely-related species unexplored. These unknowns hamper rational engineering of Pir- based surface display, a technique with the potential to convert the entire yeast cell wall into enzymatically active self-regenerating living material. To address this issue, we used a deep- learning Alphafold2-based algorithm to in silico predict the 3D protein structures of S. cerevisiae Pir proteins and compare them with structures deposited in the Protein Data Bank (PBD). Through this approach, we were able to infer proteins’ function solely through 3D homology. Moreover, we searched through the genomes of 77 species closely related to S. cerevisiae and identified over 300 Pir homologues, with which we mapped Pir evolution throughout the last 110 million years. Finally, by comparing homologous genomic loci, we show that S. cerevisiae is the only species within the Saccharomyces clade to lose one of its Pir proteins. As such, our findings point towards the role and diversity of Pir proteins in budding yeasts, thus opening novel avenues in yeast surface engineering.
Pir proteins, Alphafold2, in silico prediction of protein structures
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Podaci o prilogu
165-165.
2022.
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objavljeno
Podaci o matičnoj publikaciji
HDBMB22 From Science to Knowledge
Dulić, Morana ; Sinčić, Nino ; Vrhovac Madunić, Ivana
Zagreb: Hrvatsko Društvo za Biotehnologiju
1847-7836
Podaci o skupu
Congress of the Croatian Society of Biochemistry and Molecular Biology: From Science to Knowledge (HDBMB22)
poster
05.07.2022-07.07.2022
Brela, Hrvatska